GAG Assay Kits
Heparan Sulfate ELISA Kit
- Reagent Preparations
Transfer required volume of the reagents to sterile tubes, beakers, bottles, or other appropriate container prior to use. All the reagents including prepared reagents for the assay should be used at room temperature (RT: 15-25 °C).
[ Reaction Buffer ]
Reaction Buffer Concentrate (H); Make a 5-fold dilution in distilled water.

[ Washing Solution ]
Washing Solution Concentrate (I); Make a 5-fold dilution in distilled water.

[ Biotinylated-Antibody Solution ]
Dissolve completely Biotinylated-Antibody (Lyophilized) (D) with whole volume of the Reconstitution Buffer (E) using appropriate pipette.

[ Heparan Sulfate Standard Solutions ]
[STD range; 8-0.25µg/mL and blank (0µg/mL) ] Dilute the HS Standard (8µg/mL) (B) with the Reaction Buffer to make 5 standards (4, 2, 1, 0.5, 0.25µg/mL) . Use the Reaction Buffer as blank HS Solution (0µg/mL).

[ Other Reagents ]
Other kit components are ready to use.

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