| - Summary of Assay Procedure |
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| Step1 |
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Primary Reaction: 2-8°C, 18-24hrs. | ||
| Step2 |
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Secondary Reaction: Room Temp, 60 min. | ||
| Step3 |
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Color Development : Room Temp. in dark 30 min. | ||
| Step4 |
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| Step5 |
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Calculation of Heparan Sulfate Concentrations | |
| *Each assay step above should be performed without interruption | |||
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