
| - General Description |
| Range | 0.25-8µg/mL | |
| Measuring time | Primary Reaction | 18-24hrs |
| Secondary Reaction | 60min | |
| Color Development | 30min | |
| Blank OD (0µg/mL) | Less than 0.15 | |
| CV (wells, lots, dates, kits) |
Less than 20% | |
| Correlation coefficient (r) (log-log quadratic curve) | More than 0.980 | |
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| To make the Heparan Sulfate standard curve (quadratic curve), plot the absorbance (blank subtracted) on the x-axis (log), and the Heparan Sulfate concentration on the y-axis (log) using graph paper or appropriate software. |