GAG Assay Kits
Heparan Sulfate ELISA Kit
- The basic performance of the kit
Accuracy of the kit
  The basic performance of the Heparan Sulfate ELISA Kit was evaluated.
The difference between wells in a plate
  Three kinds of samples (urine) were measured according to the protocol using four wells per sample (in the same plate).
 
Sample HS conc.(µg/mL)
 average
(µg/mL)		 SD
 CV(%)
Well 1 Well 2 Well 3 Well 4
1 1.94 2.02 2.13 2.25 2.09 0.13 6.2
2 1.56 1.65 1.84 1.88 1.73 0.15 8.7
3 3.37 3.62 4.00 3.85 3.71 0.28 7.5
  Average CV (%) 7.5
Difference between kits
  Three kinds of samples (urine) were measured according to the protocol using three kits (from the same lot).
 
Sample
 HS conc.(µg/mL)
 Average
(µg/mL)
 SD
 CV(%)
Kit #1 Kit #2 Kit #3
1 2.09 1.90 2.16 2.05 0.13 6.3
2 1.73 1.64 1.72 1.70 0.05 2.9
3 3.71 3.63 3.83 3.72 0.10 2.7
  Average CV (%) 4.0
The difference between measurement dates.
  Three kinds of samples (urine) were measured on four different days according to the protocol using the kit (from the same lot).
 
Sample
 HS conc.(µg/mL)
 Average
(µg/mL)
 SD
 CV(%)
Day 1 Day 2 Day 3 Day 4
1 1.21 1.32 1.43 1.32 1.32 0.09 6.8
2 2.98 3.38 3.10 3.15 3.15 0.17 5.4
3 1.49 1.64 1.72 1.65 1.63 0.10 6.1
  Average CV (%) 6.1
The difference between lots.
  Three kinds of samples (urine) were measured according to the protocol using the kit (three different lots). Measurement was carried out on the same day.
 
Sample
 HS conc.(µg/mL)
 Average
(µg/mL)
 SD
 CV(%)
Lot.#1 Lot.#2 Lot.#3
1 1.66 1.46 1.41 1.51 0.13 8.6
2 1.72 1.86 1.63 1.74 0.12 6.9
3 6.51 7.34 6.75 6.87 0.43 6.3
  Average CV (%) 7.3
Conclusion
  The CV values are less than 10% in each of the above evaluations demonstrating good reproducibility of the kit.

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