| - The reactivity of the kit-1 |
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Specificity of Heparan Sulfate: Intersection reactivity with various kinds of GAGs |
| Reactivity of various kinds GAGs was evaluated with the Heparan Sulfate ELISA Kit. |
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Method | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Three-fold dilution series at 7 concentrations [180.0, 60.0, 20.0, 6.67, 2.22, 0.74 or 0.25 µg/mL] were prepared using the GAGs solution (180µg/mL) shown in the following table and they were measured according to the protocol. OD data are shown in the graph. For Hyaluronic acid, Chondroitin sulfate A, B, C, D, E, and Keratan sulfate, the influence of endogenous Heparan sulfate was minimized by Heparitinase digestion, prior to preparation of the GAG solutions. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[GAGs] |
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Result |
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Conclusion |
| The Heparan Sulfate ELISA Kit which reacts with human urine origin Heparan sulfate (UHS) and Bovine kidney origin Heparan sulfate (BKHS) was evaluated with solutions of Heparin, Hyaluronic acid, Chondroitin Sulfate A, B, C, D, and Keratan sulfate in the range 180-0.25µg/mL. Although some reactivity was found in the high concentration solutions (more than 20µg /mL) of Chondroitin sulfate E, it was about 1/100 UHS and about 1/1,000 BKHS and therefore a very weak reaction. Thereby, the Heparan Sulfate ELISA Kit has been shown to be essentially unaffected by other GAGs. |
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