GAG Assay Kits | Biochemicals | SEIKAGAKU BIOBUSINESS CORPORATION

- The reactivity of the kit-2

	Heparitinase digestive examination
	By Heparitinase digestive examination, the specificity of the Heparan sulfate in the various samples was checked with the Heparan Sulfate ELISA Kit.

Method

At room temperature (15-25oC), the samples prepared was measured according to the protocol, prior to following heparitinase digestion for 2 hours. For the serum sample, Heparitinase digestion was performed after the serum had been pretreated with Actinase E.

[GAGs]

	HSase I (-)	HSase I (+)
Sample	9 vol.	9 vol.
HSase I (1U/mL)	-	1 vol.
Reaction Buffer	1 vol.	-

Result

^*Culture Supernatant: RAW 264.7 (Abelson murine leukemia virus-induced tumor)
^**Culture Medium: 10% FBS in alpha- MEM

	Conclusion
	All samples had significant reactivity (OD value) prior to heparitinase digestion. However, this disappeared almost completely following the enzyme digestion with Heparitinase I as measured by the Heparan Sulfate ELISA Kit.

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