| - Factors which affects measurement-1 |
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Dilution linearity examination |
| The dilution linearity of various samples was checked using the Heparan Sulfate ELISA Kit. |
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Method |
| Two double dilution series of each of the samples assayed: 5 concentrations [1 (undiluted solution), 1/2, 1/4, 1/8, 1/16] were prepared and measured according to the protocol. The Heparan sulfate concentrations obtained are shown in the graph and the associated correlation coefficient correlation coefficients (r). For the serum sample, the dilution operation was performed after serum pretreatment with Actinase E. |
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Result |
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| * Culture Supernatant: RAW 264.7 (Abelson murine leukemia virus-induced tumor) **Culture Medium: 10% FBS in alpha- MEM |
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Conclusion |
| Good dilution linearity was obtained for each sample with correlation coefficients (r) in the range 0.974-1.000. |
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