GAG Assay Kits
Heparan Sulfate ELISA Kit
- Factors which affects measurement-2
Recovery Test
Method
 
A: Addition group Sample 9 Vol. + HS standard 1 Vol.
B: Non-addition group Sample 9 Vol. + Buffer 1 Vol.
C: Addition volume Buffer 9 Vol. + HS standard 1 Vol.
Result
 
Sample   Dilution A:Addition group (µg/mL) B: Non-addition group
(µg/mL)		 Recovery Vol.
A-B
(µg/mL)		 C: Addition volume
(µg/mL)		 Recovery
(%)
Urine Rat x 1/2 5.94 2.93 3.01 3.04 99.0
Human x 1 6.74 4.83 1.91 1.94 98.5
Pretreated serum Mouse x 1 1.65 0.61 1.04 0.98 106.1
Rat x 1/2 2.02 1.24 0.78 0.85 91.8
Human x 1 5.10 1.81 3.29 2.80 117.5
Culture media Cell cultured media x 1 6.38 3.30 3.08 3.04 101.3
Medium x 1 1.80 0.94 0.86 0.90 95.6

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