GAG Assay Kits
Heparan Sulfate ELISA Kit
The Measurement of heparan sulfate in serum
This pretreatment is required in measuring of Heparan Sulfate (HS) in serum from mouse, rat, human and others. This pretreatment is based on digestion of serum proteins by proteinase (actinase E).
"Heparan Sulfate ELISA Kit" does not contain reagents of this pretreatment.

- Method of pretreatment
Instruments and Reagents Required
  • Actinase E (KAKEN pharmaceutical CO., LTD. JAPAN)
  • Actinase E Dissolution Buffer (0.5M Tris-HCl, pH7.2-7.5)
  • Water Bath (55°C)
  • Centrifugate
  • Mixer
  • Centrifuge tube or test tube with screw cap (made from polypropylene)*1
    *1:The tube with screw cap should be airtight avoid to evaporate of sample or inflow water.
Preparation of Reagents
All the reagents prepare before use. Prepared reagents should use promptly and avoid to storage.

[ Actinase E Dissolution Buffer (0.5M Tris-HCl buffer, pH7.2-7.5) ]
  1. Put 6.06g of Tris (hydroxymetyl) aminomethane into 100mL beaker, add 40mL of distilled water (DW), and then dissolve completely.
  2. Adjust to pH7.2-7.5 by using 1N HCl.
  3. Transfer this solution to 100mL graduated cylinder, fill up to 100mL with DW and then mix well.
[ Actinase E Solution ]
Dissolve completely actinase E (20mg/mL) in Actinase E Dissolution Buffer.

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