| Step1 |
| Standard Solution;
50µL or Samples; 50µL |
|
|
| |
 |
Primary Reaction:37°C, 60 min. |
| Step2 |
HRP-Conjugated Streptavidin Solution; 25µL
+
Biotinylated Antibody Solution; 25µL |
|
|
| |
|
Secondary Reaction:37°C, 60 min. |
| Step3 |
|
|
| |
|
Development Reaction: Room Temp. in
dark, 10 min. |
| Step4 |
|
|
| |
|
|
| Step5 |
| Measure the absorbance at 450nm/630nm |
|
Calculation of Keratan Sulfate Concentrations |
