GAG Assay Kits
High Sensitive Keratan Sulfate ELISA Kit
Technical information: High Sensitive Keratan Sulfate ELISA Kit

- Basic Performance

[ Accuracy of High Sensitive Keratan Sulfate ELISA Kit ]

The basic performance of the High Sensitive Keratan Sulfate ELISA Kit was evaluated.

Difference between wells on the same plate

4 types of sample (diluted serum) were measured in accordance with the protocol using six wells per sample (on the same plate).

Sample Measured value (ng/mL) Average value (ng/mL) SD C.V. (%)
Well 1 Well 2 Well 3 Well 4 Well 5 Well 6
1 2.21 2.16 2.37 2.33 2.70 2.40 2.36 0.19 8.0
2 1.70 1.56 1.57 1.67 1.67 1.56 1.62 0.06 4.0
3 0.89 0.81 0.75 0.78 0.80 0.74 0.80 0.05 6.8
4 0.47 0.46 0.41 0.41 0.44 0.38 0.43 0.03 8.0
  Average C.V. (%) 6.7

Difference between kits

4 types of sample (diluted serum) were measured in accordance with the protocol by using four kits (from the same lot).

Sample Measured value (ng/mL) Average value (ng/mL) SD C.V. (%)
Kit 1 Kit 2 Kit 3 Kit 4
1 2.43 2.26 2.55 2.52 2.44 0.13 5.3
2 1.64 1.48 1.70 1.68 1.63 0.10 6.3
3 0.79 0.75 0.84 0.84 0.81 0.04 5.3
4 0.43 0.42 0.48 0.44 0.44 0.03 6.1
  Average C.V. (%) 5.7

Difference when measurements carried out on different dates

4 types of sample (diluted serum) were measured in accordance with the protocol by using the kits (from the same lot) on four different dates.

Sample Measured value (ng/mL) Average value (ng/mL) SD C.V. (%)
Day 1 Day 2 Day 3 Day 4
1 2.26 2.52 2.36 2.63 2.44 0.16 6.6
2 1.48 1.69 1.62 1.71 1.62 0.10 6.3
3 0.75 0.83 0.80 0.85 0.81 0.04 5.3
4 0.42 0.41 0.43 0.45 0.42 0.02 4.1
  Average C.V. (%) 5.6

Conclusion

CV values were <10% in each of the above evaluations, demonstrating good reproducibility within plates, between kits, and on different dates.


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