Method

For the High Sensitive Kit, a 1/4 dilution series of seven concentrations (1,000, 250, 62.5, 15.63, 3.91, 0.8, and 0.24 ng) was prepared of 1,000 ng/mL solutions of the keratan sulfate listed in the table below. For the conventional kit, a 1/2 dilution series of seven concentrations (2,000, 1,000, 500, 250, 125, 62.5, and 31.25 ng) of 2,000 ng/mL bovine cornea keratan sulfate and a 1/2 dilution series of seven concentrations (100, 50, 25, 12.5, 6.25, 3.13., and 1.56 ng/mL) of 100 ng/mL shark cartilage keratan polysulfate were prepared. These keratan sulfate solutions were measured according to the protocols for each kit, and the resulting absorbances were shown on a graph.

Results

Conclusion

Both the High Sensitive and conventional kits were confirmed to exhibit greater reactivity with keratan polysulfate derived from shark cartilage (KPS) than with keratan sulfate derived from bovine cornea (KSBC). The sensitivity of the High Sensitive kit for keratan sulfate was approximately 10 times that of the conventional kit for KPS and 100 times for KSBC, detecting both types of keratan sulfate with greater sensitivity
The conventional kit is considered to preferentially measure keratan sulfate with a higher level of sulfation, whereas the High Sensitive kit measures the keratin sulfate present in samples with greater sensitivity.