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 Technical Information: Sulfated GAG Quantitation Kit |
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| - General Description |
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[ Principle of the Sulfated GAG Quantitation Kit ]
DMMB dye alone in solution exhibits maximum absorbance at 590 nm, but when complexes with sulfated GAGs are formed, absorbance at 590 nm declines and an absorbance peak is generated at 530 nm. This phenomenon can be used to enable simple measurement of sulfated GAG concentrations.
[ Kit overview ]
| Range | 2.5-80µg/mL | |
| Time required | Pre-treatment | 2-20 hrs. |
| Assay | 5 min | |
| C.V. (within plates, between kits, between measurement days, between lots) |
< 10% | |
| Correlation coefficient (r) (linear-linear, quadratic curve) |
> 0.99 | |
[ Typical standard curve ]
Produce a standard curve (quadratic curve) by plotting sulfated GAG standard concentrations on the x-axis and absorbance values on the y-axis.
If using Microsoft Excel, convert the sulfated GAG standard solution concentrations to mg/mL and plot these on the x-axis with the absorbance on the y-axis to produce a scatterplot graph. Calculate the quadratic function of the approximation curve, derive the inverse function, and substitute the absorbance of the test sample on the y-axis. Derive the x-value and convert to micrograms per milliliter.
*An Excel conversion sheet for use with this method is available. Please contact us if you would like to request a copy.
