GAG Assay Kits
Sulfated Glycosaminoglycan Quantitation Kit
Technical Information: Sulfated GAG Quantitation Kit

- Factors Affecting Measurements (1)

[ Dilution Linearity Examination ]

We used the Sulfated GAG Quantitation Kit to verify test the dilution linearity of different types of sample.

Method

A 1/2 dilution series (concentrations: original, 1/2, 1/4, 1/8) was prepared of the types of sample listed below, and measurements carried out in accordance with the protocol. The resulting sulfated GAG concentrations were indicated on a graph, and the correlation coefficients derived.

Results

Dilution linearity (Pig Knee Cartilage) Dilution linearity (Rat Dorsal Skin) Dilution linearity (pig chondrocyte culture solution 1) Dilution linearity (pig chondrocyte culture solution 2)

Conclusion

The correlation coefficient (r) for each type of sample was 0.991-1.000, verifying that good dilution linearity is displayed.