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 HABP:Hyaluronic Acid Binding Protein |
| B-HABP:Biotinylated HABP |
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| - SDS-PAGE |
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| [Method of SDS-PAGE] | |||||||||||||||||||||
| The figure shows analyses of HABP which was subjected to electrophoresis on SDS-polyacrylamide gel (7.5% acrylamide) before or after treatment with keratanase. | |||||||||||||||||||||
| [Result] | |||||||||||||||||||||
| HABP gave 2-3 broad bands (70-80kDa, 45kDa and 40-45kDa) that can be bound specifically to hyaluronic acid and keratanase treatment HABP gave 2-3 broad bands (70kDa, 45kDa and 40-45kDa). That suggests that component (70-80kDa) of HABP may contain keratan sulfate. Therefore, HABP may consist of 3 fragments (first with keratan sulfate, second without keratan sulfate and third, link protein) that are present in proteoglycan aggregate preparation. | |||||||||||||||||||||
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