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The distribution patterns of gangliosides during postnatal development of rat cerebellar cortex

Age	Ganglioside
Layer of cerebellar cortex	GM1	GD1a	GD1b	GT1b	GQ1b	GM3	GD3/ LD1a	GD2	O-Ac GD3	O-Ac LD1
1 day
Ext. granular layerb	++^d		++				+++		++
Molecular layer	+	+	+	+			+
Purkinje cell layer
Int. granular layerc	++	+	+	+			++
White matter

5 day
Ext. granular layer	++		++				+++	+	+++
Molecular layer	++	++	+	+			+	+	++
Purkinje cell layer										+
Int. granular layer	+	+	+	+			++	+
White matter

10 day
Ext. granular layer	++		++			+	+++	+	+++
Molecular layer	+	++	+	+	+	+	+	+	+
Purkinje cell layer										+
Int. granular layer	+	++	++	++	+		++	+
White matter

20 day
Ext. granular layer	+				+		++		++
Molecular layer	+	++	+	+	+	+	+	+	+	+
Purkinje cell layer										++
Int. granular layer	+	+	++	++			++	++
White matter	+		++	+++		++	++	+

30 day
Molecular layer	+	++	+	+	+	+	+	+		++
Purkinje cell layer										+++
Granular layer	+	+	++	+++	++	+	+++	+
White matter	+		+	++		+++	+++

Adult
Molecular layer	+	+++	+	++				+		+++
Purkinje cell layer										+++
Granular layer	+	+	+++	+++	++	+	++	+++
White matter	+++			+		+++	++
a MAb GMR19 reacts with both GD3 and LD1. GD3 was detected from adulthood, whereas LD1 was expressed at later postnatal days. b Ext. granular layer, external granular layer. c Int. granular layer, internal granular layer. d Four grade have been assigned to each ganglioside labeling density; +++, strong; ++, moderate; +, weak; -, negative.