Monoclonals | Biochemicals | SEIKAGAKU BIOBUSINESS CORPORATION

Distribution of glycolipids in rat brain by monoclonal antibodies

- Distribution of major gangliosides in rat nervous system detected by monoclonal antibodies

Rat region	Ganglioside
	GM1	GD1a	GD1b	GT1b	GQ1b
Cerebellar cortex
Molecular layer	+	+++	+	++
Purkinje cell layer
Granular layer	+	+	+++	+++	++
White matter	+++

Cerebral cortex
Molecular layer (1)	+	+++		+
Ext. granular layer (II)	+	+++	+
Ext. pyramidal layer (III)	+	+++	++	++
Int. granular layer (IV)	+		+++	+	+
Int. pyramidal layer (Va)	+	+	+++	++
Int. pyramidal layer (Vb)	+		+++	++	+
Polymorphic cell layer(VI)	+	+	+++	+	+
White matter	++		+	+++

Hippocampal formation
Hippocampus
Alveus	++		++	++	+++
Stratum oriens	++	++		+	+
Stratum pyramidale			+		++
Stratum radiatum		++		+
Stratum lacunosummolelulare	++		++	+
Dentate gyrus
Polymorphic layer	+	++	+	+
Granular layer			++
Molecular layer	+	++		++

Spinal cord
Gray matter
Anterior horn	+		+++	+++	++
Posterior horn	+	+++	+++	+++	++
White matter
Anterior funiculus	+		+	++
Lateral funiculus	+		+	++
Posterior funiculus	+		+	++
1Gangliosides GM1, GD1a, GD1b, GT1b, and GQ1b were detected by five MAbs GMB16, GMR17, GGR12, GMR5, and GMR13, respectively, with indirect immunofluorecence technique. 2+++, strong; ++, moderate; +, weak; - , negative Kotani, M. et al . : Glycobiology,* 4, 855-865(1994)